Polyclonal Antibody to Histone H3 (H3)
- Product No.PAA285Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 258
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Bovine Cerebrum Tissue; Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Bovine Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Bovine Spinal cord Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Bovine Cerebellum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Bovine Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Bovine Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Bovine Small intestine Tissue
Primary Ab: 20µg/ml Rabbit Anti-Bovine H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Bovine Cerebellum Tissue
Primary Ab: 20µg/ml Rabbit Anti-Bovine H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Bovine Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Bovine H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Bovine H3 Antibody Second A
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Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Kidney lysate; Lane3: Rat Stomach lysate; Lane4: Mouse Liver lysate; Lane5: Mouse Kidney lysate; Lane6: Mouse Stomach lysate
Primary Ab: 0.05µg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Spleen lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Histone H3 (H3)
The antibody is a rabbit polyclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Histone H3 (H3)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Histone H3 (H3)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Histone H3 (H3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Related products
| Catalog No. | Organism species: Bos taurus; Bovine (Cattle) | Applications (RESEARCH USE ONLY!) |
| RPA285Bo01 | Recombinant Histone H3 (H3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA285Bo01 | Polyclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
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