Polyclonal Antibody to Complement C3 Convertase (C3 Convertase)
C4b2a; C3bBb, complement C.hivin.4.hivin2
- Product No.PAA758Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA758Mu01-Recombinant Complement C3 Convertase (C3 Convertase)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Mouse Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse C3 Convertase Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Testis Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse C3 Convertase Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse C3 Convertase Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse C3 Convertase Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant C3c, Mouse.
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Figure. Western Blot; Sample: Lane1: Mouse Serum; Lane2: Human Serum; Lane3: Mouse Lung Tissue; Lane4: Mouse Heart Tissue; Lane5: Human HepG2 Cells.
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HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-mouse C3c Polyclonal Antibody (Catalog # PAA758Mu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Complement C3 Convertase (C3 Convertase)
The antibody is a rabbit polyclonal antibody raised against C3 Convertase. It has been selected for its ability to recognize C3 Convertase in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Complement C3 Convertase (C3 Convertase)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Complement C3 Convertase (C3 Convertase)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Complement C3 Convertase (C3 Convertase)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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