Mouse Model for Liver Failure (LF)
Hepatic failure
- Product No.DSI556Mu03
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Prototype SpeciesHuman
- Sourceinduced by carbon tetrachloride
- Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 8~10weeks, body weight:30g~35g.
- Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
- Modeling Period12~14 weeks
- Modeling MethodModeling method:
Establishment of chronic liver injury model in mice: BALB/ C mice, male, random grouping. Except for normal control group, other mice were intraperitoneally injected 20% CCl4 oil solution (5mL /kg) twice a week for 12 weeks. D-Gal (1g/kg) and LPS (10ug/kg) were intraperitoneally injected after 12 weeks of administration for 3 days.
Intervention administration: TSA (Trichostatin A), sodium n-butyrate, NF-κB inhibitor (PDTC) were administered respectively. - Applications疾病模型
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Model Evaluation of the Mouse Model for Liver Failure (LF)
After modeling, the mice in each group were sacrificed, and the blood was collected and the serum was separated for biochemical detection. Liver and intestinal tissues were collected for pathological examination.
1. Serum biochemical indexes ALT and AST were detected;
2. Liver tissue was taken, fixed, dehydrated and embedded, and paraffin section was prepared.
HE staining and TUNEL fluorescence staining were performed respectively.
3. Intestinal tissue was taken for HE staining.
Intestinal tissue (small intestine) was taken, fixed and embedded, paraffin section prepared, HE staining.
4. Serum was separated and the levels of HMGB1, TNF-α and IL-18 in each group were detected by ELISA.
Pathological Results of the Mouse Model for Liver Failure (LF)
Cytokines Level of the Mouse Model for Liver Failure (LF)
The levels of HMGB1, TNF-α and IL-18 in serum samples were detected by ELISA.
Statistical Analysis of the Mouse Model for Liver Failure (LF)
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
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INCREMENT SERVICES
Tissue/Sections Customized Service
Serums Customized Service
Immunohistochemistry (IHC) Experiment Service
Small Animal In Vivo Imaging Experiment Service
Small Animal Micro CT Imaging Experiment Service
Small Animal MRI Imaging Experiment Service
Small Animal Ultrasound Imaging Experiment Service
Transmission Electron Microscopy (TEM) Experiment Service
Scanning Electron Microscope (SEM) Experiment Service
Learning and Memory Behavioral Experiment Service
Anxiety and Depression Behavioral Experiment Service
Drug Addiction Behavioral Experiment Service
Pain Behavioral Experiment Service
Neuropsychiatric Disorder Behavioral Experiment Service
Fatigue Behavioral Experiment Service
Nitric Oxide Assay Kit (A012)
Nitric Oxide Assay Kit (A013-2)
Total Anti-Oxidative Capability Assay Kit(A015-2)
Total Anti-Oxidative Capability Assay Kit (A015-1)
Superoxide Dismutase Assay Kit
Fructose Assay Kit (A085)
Citric Acid Assay Kit (A128 )
Catalase Assay Kit
Malondialdehyde Assay Kit
Glutathione S-Transferase Assay Kit
Microscale Reduced Glutathione assay kit
Glutathione Reductase Activity Coefficient Assay Kit
Angiotensin Converting Enzyme Kit
Glutathione Peroxidase (GSH-PX) Assay Kit
Cloud-Clone Multiplex assay kits
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DSI556Mu02 | Mouse Model for Liver Failure (LF) | n/a |
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DSI556Mu04 | Mouse Model for Liver Failure (LF) | n/a |
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