Monoclonal Antibody to Histone H3 (H3)
- Product No.MAA285Mi21
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C4
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA285Mi01-Recombinant Histone H3 (H3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: U2OS cell lysate Primary Ab: 0.2μg/ml Mouse Anti-Multi-species H3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Bovine Cerebrum lysate; Lane2: Rat Liver lysate; Lane3: Mouse Cerebrum lysate; Lane4: Canine Cerebrum lysate
Primary Ab: 0.2µg/ml Mouse Anti-Multi-species H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Histone H3 (H3)
The antibody is a mouse monoclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Histone H3 (H3)
Western blotting: 0.2-2µg/mL
Immunohistochemistry: 2-20µg/mL
Immunocytochemistry: 2-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Histone H3 (H3)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Histone H3 (H3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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