Mini Samples ELISA Kit for Triiodothyronine (T3)

3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

  • Mini Samples ELISA Kit for Triiodothyronine (T3) Packages (Simulation)
  • Mini Samples ELISA Kit for Triiodothyronine (T3) Packages (Simulation)
  • Mini Samples ELISA Kit for Triiodothyronine (T3) Results demonstration
  • MEA453Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Mini Samples ELISA Kit for Triiodothyronine (T3)

This assay has high sensitivity and excellent specificity for detection of Mini Samples Triiodothyronine (T3).
No significant cross-reactivity or interference between Mini Samples Triiodothyronine (T3) and analogues was observed.

Recovery of the Mini Samples ELISA Kit for Triiodothyronine (T3)

Matrices listed below were spiked with certain level of Mini Samples Triiodothyronine (T3) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Triiodothyronine (T3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-94 86
EDTA plasma(n=5) 83-102 95
heparin plasma(n=5) 83-96 86

Precision of the Mini Samples ELISA Kit for Triiodothyronine (T3)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Triiodothyronine (T3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Mini Samples ELISA Kit for Triiodothyronine (T3)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Triiodothyronine (T3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 78-99% 80-98% 95-103%
EDTA plasma(n=5) 96-103% 94-102% 87-101% 91-99%
heparin plasma(n=5) 89-102% 98-105% 82-94% 89-98%

Stability of the Mini Samples ELISA Kit for Triiodothyronine (T3)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Mini Samples ELISA Kit for Triiodothyronine (T3)

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Test principle of the Mini Samples ELISA Kit for Triiodothyronine (T3)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Triiodothyronine (T3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Triiodothyronine (T3) and unlabeled Mini Samples Triiodothyronine (T3) (Standards or samples) with the pre-coated antibody specific to Mini Samples Triiodothyronine (T3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Triiodothyronine (T3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Triiodothyronine (T3) in the sample.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
CPA453Ge11 BSA Conjugated Triiodothyronine (T3) Immunogen; SDS-PAGE; WB.
CPA453Ge21 OVA Conjugated Triiodothyronine (T3) Immunogen; SDS-PAGE; WB.
PAA453Ge01 Polyclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA453Ge71 Biotin-Linked Polyclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC.
MAA453Ge21 Monoclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC; IP.
MAA453Ge23 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge22 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge24 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge25 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge27 Monoclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC; IP.
LAA453Ge72 Biotin-Linked Monoclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC.
CEA453Ge ELISA Kit for Triiodothyronine (T3) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA453Ge Instant ELISA Kit for Triiodothyronine (T3) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA453Ge Mini Samples ELISA Kit for Triiodothyronine (T3) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA453Ge ELISA Kit for Anti-Triiodothyronine Antibody (Anti-T3) Enzyme-linked immunosorbent assay for Antibody Detection.
CCA453Ge CLIA Kit for Triiodothyronine (T3) Chemiluminescent immunoassay for Antigen Detection.
LMA453Ge Multiplex Assay Kit for Triiodothyronine (T3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA453Ge11 ELISA Kit DIY Materials for Triiodothyronine (T3) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK