Mini Samples ELISA Kit for Caspase 9 (CASP9)
APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6
- Product No.MEA627Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.129ng/mL.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 529
For more details, please contact local distributors!US$ 756
For more details, please contact local distributors! US$ 3402
For more details, please contact local distributors! US$ 6426
For more details, please contact local distributors! US$ 52920
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Caspase 9 (CASP9)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Caspase 9 (CASP9).
No significant cross-reactivity or interference between Mini Samples Caspase 9 (CASP9) and analogues was observed.
Precision of the Mini Samples ELISA Kit for Caspase 9 (CASP9)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Caspase 9 (CASP9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Caspase 9 (CASP9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability of the Mini Samples ELISA Kit for Caspase 9 (CASP9)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Caspase 9 (CASP9)
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle of the Mini Samples ELISA Kit for Caspase 9 (CASP9)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Caspase 9 (CASP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Caspase 9 (CASP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Caspase 9 (CASP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Caspase 9 (CASP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA627Hu04 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA627Hu03 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA627Hu01 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA627Hu02 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA627Hu02 | Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| PAA627Hu03 | Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| PAA627Hu01 | Polyclonal Antibody to Caspase 9 (CASP9) | WB |
| LAA627Hu81 | FITC-Linked Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IF. |
| LAA627Hu71 | Biotin-Linked Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC. |
| MAA627Hu23 | Monoclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| MAA627Hu27 | Monoclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| MAA627Hu29 | Monoclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| MAA627Hu21 | Monoclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| MAA627Hu24 | Monoclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| MAA627Hu25 | Monoclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| MAA627Hu22 | Monoclonal Antibody to Caspase 9 (CASP9) | WB,IHC |
| MEA627Hu | Mini Samples ELISA Kit for Caspase 9 (CASP9) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA627Hu | ELISA Kit for Caspase 9 (CASP9) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA627Hu | Multiplex Assay Kit for Caspase 9 (CASP9) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA627Hu01 | ELISA Kit DIY Materials for Caspase 9 (CASP9) | Main materials for "Do It (ELISA Kit) Yourself". |