Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

5HT; Serotonin; N-Methyl-Gamma

  • Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT) Packages (Simulation)
  • Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT) Packages (Simulation)
  • Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT) Results demonstration
  • MEA808Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

This assay has high sensitivity and excellent specificity for detection of Mini Samples 5-Hydroxytryptamine (5-HT).
No significant cross-reactivity or interference between Mini Samples 5-Hydroxytryptamine (5-HT) and analogues was observed.

Recovery of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

Matrices listed below were spiked with certain level of Mini Samples 5-Hydroxytryptamine (5-HT) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples 5-Hydroxytryptamine (5-HT) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 80-96 92

Precision of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples 5-Hydroxytryptamine (5-HT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples 5-Hydroxytryptamine (5-HT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples 5-Hydroxytryptamine (5-HT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 83-93% 85-102% 81-92%
EDTA plasma(n=5) 98-105% 78-98% 86-98% 80-95%
heparin plasma(n=5) 81-101% 90-105% 94-105% 84-99%

Stability of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Test principle of the Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples 5-Hydroxytryptamine (5-HT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples 5-Hydroxytryptamine (5-HT) and unlabeled Mini Samples 5-Hydroxytryptamine (5-HT) (Standards or samples) with the pre-coated antibody specific to Mini Samples 5-Hydroxytryptamine (5-HT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples 5-Hydroxytryptamine (5-HT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples 5-Hydroxytryptamine (5-HT) in the sample.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
CPA808Ge11 BSA Conjugated 5-Hydroxytryptamine (5-HT) Immunogen; SDS-PAGE; WB.
CPA808Ge21 OVA Conjugated 5-Hydroxytryptamine (5-HT) Immunogen; SDS-PAGE; WB.
PAA808Ge01 Polyclonal Antibody to 5-Hydroxytryptamine (5-HT) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA808Ge71 Biotin-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT) WB; IHC; ICC.
LAA808Ge81 FITC-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT) WB; IHC; ICC; IF.
MEA808Ge Mini Samples ELISA Kit for 5-Hydroxytryptamine (5-HT) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA808Ge ELISA Kit for 5-Hydroxytryptamine (5-HT) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA808Ge Instant ELISA Kit for 5-Hydroxytryptamine (5-HT) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA808Ge ELISA Kit for Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) Enzyme-linked immunosorbent assay for Antibody Detection.
CCA808Ge CLIA Kit for 5-Hydroxytryptamine (5-HT) Chemiluminescent immunoassay for Antigen Detection.
LMA808Ge Multiplex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA808Ge11 ELISA Kit DIY Materials for 5-Hydroxytryptamine (5-HT) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK