High Sensitive ELISA Kit for Complement Component 4d (C4d)

  • High Sensitive ELISA Kit for Complement Component 4d (C4d) Packages (Simulation)
  • High Sensitive ELISA Kit for Complement Component 4d (C4d) Packages (Simulation)
  • High Sensitive ELISA Kit for Complement Component 4d (C4d) Results demonstration
  • HES103Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

This assay has high sensitivity and excellent specificity for detection of High Sensitive Complement Component 4d (C4d).
No significant cross-reactivity or interference between High Sensitive Complement Component 4d (C4d) and analogues was observed.

Recovery of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

Matrices listed below were spiked with certain level of recombinant High Sensitive Complement Component 4d (C4d) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Complement Component 4d (C4d) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 99
EDTA plasma(n=5) 93-101 98
heparin plasma(n=5) 78-88 82

Precision of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Complement Component 4d (C4d) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Complement Component 4d (C4d) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Complement Component 4d (C4d) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 84-92% 78-91% 96-105%
EDTA plasma(n=5) 88-97% 97-105% 85-97% 81-94%
heparin plasma(n=5) 92-99% 97-105% 87-101% 90-104%

Stability of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the High Sensitive ELISA Kit for Complement Component 4d (C4d)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Complement Component 4d (C4d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Complement Component 4d (C4d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Complement Component 4d (C4d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Complement Component 4d (C4d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPS103Hu01 Recombinant Complement Component 4d (C4d) Positive Control; Immunogen; SDS-PAGE; WB.
PAS103Hu01 Polyclonal Antibody to Complement Component 4d (C4d) WB; IHC; ICC; IP.
MAS103Hu22 Monoclonal Antibody to Complement Component 4d (C4d) WB; IHC; ICC; IP.
MAS103Hu23 Monoclonal Antibody to Complement Component 4d (C4d) WB; IHC; ICC; IP.
MAS103Hu21 Monoclonal Antibody to Complement Component 4d (C4d) WB; IHC; ICC; IP.
SES103Hu ELISA Kit for Complement Component 4d (C4d) Enzyme-linked immunosorbent assay for Antigen Detection.
HES103Hu High Sensitive ELISA Kit for Complement Component 4d (C4d) Enzyme-linked immunosorbent assay for Antigen Detection.
SCS103Hu CLIA Kit for Complement Component 4d (C4d) Chemiluminescent immunoassay for Antigen Detection.
LMS103Hu Multiplex Assay Kit for Complement Component 4d (C4d) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK