ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

  • ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Results demonstration
  • CEA124Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.

Recovery of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 1 (TGFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 1 (TGFb1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 97
EDTA plasma(n=5) 85-101 97
heparin plasma(n=5) 91-104 97

Precision of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 1 (TGFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 89-97% 90-97% 86-101%
EDTA plasma(n=5) 86-104% 90-97% 93-105% 78-98%
heparin plasma(n=5) 89-97% 99-105% 95-103% 85-104%

Stability of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transforming Growth Factor Beta 1 (TGFb1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Transforming Growth Factor Beta 1 (TGFb1) and unlabeled Transforming Growth Factor Beta 1 (TGFb1) (Standards or samples) with the pre-coated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Transforming Growth Factor Beta 1 (TGFb1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Transforming Growth Factor Beta 1 (TGFb1) in the sample.

Related products

Catalog No. Organism species: Bos taurus; Bovine (Cattle) Applications (RESEARCH USE ONLY!)
APA124Bo01 Active Transforming Growth Factor Beta 1 (TGFb1) Cell culture; Activity Assays.
RPA124Bo01 Recombinant Transforming Growth Factor Beta 1 (TGFb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA124Bo01 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1) WB; IHC; ICC; IP.
MAA124Bo21 Monoclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1) WB; IHC; ICC; IP.
SEA124Bo ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA124Bo ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA124Bo Multiplex Assay Kit for Transforming Growth Factor Beta 1 (TGFb1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK