ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

  • ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Packages (Simulation)
  • ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Packages (Simulation)
  • ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Results demonstration
  • CEA957Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP).
No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Recovery of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Matrices listed below were spiked with certain level of recombinant Procollagen I N-Terminal Propeptide (PINP) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen I N-Terminal Propeptide (PINP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-104 95
EDTA plasma(n=5) 94-103 99
heparin plasma(n=5) 83-92 86

Precision of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen I N-Terminal Propeptide (PINP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 90-104% 99-105% 99-105%
EDTA plasma(n=5) 78-105% 95-102% 78-88% 82-91%
heparin plasma(n=5) 88-96% 93-102% 90-97% 80-99%

Stability of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen I N-Terminal Propeptide (PINP) and unlabeled Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA957Mu01 Recombinant Procollagen I N-Terminal Propeptide (PINP) Positive Control; Immunogen; SDS-PAGE; WB.
SPA957Mu01 Synthetic Procollagen I N-Terminal Propeptide (PINP) Immunogen; Blocking Peptide.
CPA957Mu21 OVA Conjugated Procollagen I N-Terminal Propeptide (PINP) Immunogen; SDS-PAGE; WB.
PAA957Mu01 Polyclonal Antibody to Procollagen I N-Terminal Propeptide (PINP) WB; IHC; ICC; IP.
PAA957Mu08 Polyclonal Antibody to Procollagen I N-Terminal Propeptide (PINP) WB; IHC; ICC; IP.
SEA957Mu ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA957Mu Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA957Mu ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA957Mu11 ELISA Kit DIY Materials for Procollagen I N-Terminal Propeptide (PINP) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK