ELISA Kit for Presenilin 1 (PSEN1)

FAD; AD3; PS1; PSNL1; S182; Alzheimer Disease 3; Protein S182; Presenilin-1 CTF12

  • ELISA Kit for Presenilin 1 (PSEN1) Packages (Simulation)
  • ELISA Kit for Presenilin 1 (PSEN1) Packages (Simulation)
  • ELISA Kit for Presenilin 1 (PSEN1) Results demonstration
  • SEC200Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Presenilin 1 (PSEN1)

This assay has high sensitivity and excellent specificity for detection of Presenilin 1 (PSEN1).
No significant cross-reactivity or interference between Presenilin 1 (PSEN1) and analogues was observed.

Recovery of the ELISA Kit for Presenilin 1 (PSEN1)

Matrices listed below were spiked with certain level of recombinant Presenilin 1 (PSEN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Presenilin 1 (PSEN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-104 98
EDTA plasma(n=5) 80-95 87
heparin plasma(n=5) 88-95 92

Precision of the ELISA Kit for Presenilin 1 (PSEN1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Presenilin 1 (PSEN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Presenilin 1 (PSEN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Presenilin 1 (PSEN1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Presenilin 1 (PSEN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 84-104% 87-103% 96-105%
EDTA plasma(n=5) 87-102% 98-105% 91-105% 87-95%
heparin plasma(n=5) 99-105% 89-103% 85-101% 83-96%

Stability of the ELISA Kit for Presenilin 1 (PSEN1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Presenilin 1 (PSEN1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Presenilin 1 (PSEN1)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Presenilin 1 (PSEN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Presenilin 1 (PSEN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Presenilin 1 (PSEN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Presenilin 1 (PSEN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPC200Mu01 Recombinant Presenilin 1 (PSEN1) Positive Control; Immunogen; SDS-PAGE; WB.
RPC200Mu02 Recombinant Presenilin 1 (PSEN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC200Mu01 Polyclonal Antibody to Presenilin 1 (PSEN1) WB; IHC; ICC; IP.
PAC200Mu02 Polyclonal Antibody to Presenilin 1 (PSEN1) WB; IHC; ICC; IP.
SEC200Mu ELISA Kit for Presenilin 1 (PSEN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC200Mu Multiplex Assay Kit for Presenilin 1 (PSEN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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