ELISA Kit for Trypsin (TRY)

  • ELISA Kit for Trypsin (TRY) Packages (Simulation)
  • ELISA Kit for Trypsin (TRY) Packages (Simulation)
  • ELISA Kit for Trypsin (TRY) Results demonstration
  • SEA250Po.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Trypsin (TRY)

This assay has high sensitivity and excellent specificity for detection of Trypsin (TRY).
No significant cross-reactivity or interference between Trypsin (TRY) and analogues was observed.

Recovery of the ELISA Kit for Trypsin (TRY)

Matrices listed below were spiked with certain level of recombinant Trypsin (TRY) and the recovery rates were calculated by comparing the measured value to the expected amount of Trypsin (TRY) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-105 101
EDTA plasma(n=5) 80-103 99
heparin plasma(n=5) 81-91 87

Precision of the ELISA Kit for Trypsin (TRY)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trypsin (TRY) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trypsin (TRY) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Trypsin (TRY)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trypsin (TRY) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 82-90% 78-93% 81-89%
EDTA plasma(n=5) 95-105% 93-102% 80-88% 87-94%
heparin plasma(n=5) 98-105% 80-104% 94-102% 94-101%

Stability of the ELISA Kit for Trypsin (TRY)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Trypsin (TRY)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Trypsin (TRY)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trypsin (TRY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trypsin (TRY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trypsin (TRY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trypsin (TRY) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Sus scrofa; Porcine (Pig) Applications (RESEARCH USE ONLY!)
NPA250Po01 Native Trypsin (TRY) Positive Control; Immunogen; SDS-PAGE; WB.
RPA250Po01 Recombinant Trypsin (TRY) Positive Control; Immunogen; SDS-PAGE; WB.
APA250Po51 Active Trypsin (TRY) Cell culture; Activity Assays.
PAA250Po02 Polyclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
PAA250Po01 Polyclonal Antibody to Trypsin (TRY) WB
LAA250Po71 Biotin-Linked Polyclonal Antibody to Trypsin (TRY) WB; IHC; ICC.
MAA250Po21 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
MAA250Po23 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
MAA250Po24 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
MAA250Po22 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
SEA250Po ELISA Kit for Trypsin (TRY) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA250Po CLIA Kit for Trypsin (TRY) Chemiluminescent immunoassay for Antigen Detection.
LMA250Po Multiplex Assay Kit for Trypsin (TRY) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA250Po01 Antibody Pair for Trypsin (TRY) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA250Po01 ELISA Kit DIY Materials for Trypsin (TRY) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK