ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

HNAC1; non-lysosomal ceramidase; Neutral ceramidase

  • ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2) Packages (Simulation)
  • ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2) Packages (Simulation)
  • ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2) Results demonstration
  • SEE689Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

This assay has high sensitivity and excellent specificity for detection of N-Acylsphingosine Amidohydrolase 2 (ASAH2).
No significant cross-reactivity or interference between N-Acylsphingosine Amidohydrolase 2 (ASAH2) and analogues was observed.

Recovery of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

Matrices listed below were spiked with certain level of recombinant N-Acylsphingosine Amidohydrolase 2 (ASAH2) and the recovery rates were calculated by comparing the measured value to the expected amount of N-Acylsphingosine Amidohydrolase 2 (ASAH2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 82-89 85
heparin plasma(n=5) 80-101 85

Precision of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Acylsphingosine Amidohydrolase 2 (ASAH2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Acylsphingosine Amidohydrolase 2 (ASAH2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-Acylsphingosine Amidohydrolase 2 (ASAH2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-102% 96-105% 79-89% 81-94%
EDTA plasma(n=5) 90-98% 81-95% 92-99% 78-93%
heparin plasma(n=5) 94-105% 80-88% 98-105% 81-104%

Stability of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylsphingosine Amidohydrolase 2 (ASAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acylsphingosine Amidohydrolase 2 (ASAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylsphingosine Amidohydrolase 2 (ASAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylsphingosine Amidohydrolase 2 (ASAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPE689Hu01 Recombinant N-Acylsphingosine Amidohydrolase 2 (ASAH2) Positive Control; Immunogen; SDS-PAGE; WB.
APE689Hu61 Active N-Acylsphingosine Amidohydrolase 2 (ASAH2) Cell culture; Activity Assays.
EPE689Hu61 Eukaryotic N-Acylsphingosine Amidohydrolase 2 (ASAH2) Positive Control; Immunogen; SDS-PAGE; WB.
PAE689Hu01 Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2) WB; IHC; ICC; IP.
MAE689Hu21 Monoclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2) WB; IHC; ICC; IP.
SEE689Hu ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2) Enzyme-linked immunosorbent assay for Antigen Detection.
HEE689Hu High Sensitive ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2) Enzyme-linked immunosorbent assay for Antigen Detection.
LME689Hu Multiplex Assay Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK