ELISA Kit for Vitamin D Binding Protein (DBP)

GC; VDBG; VDBP; Group-Specific Component; Gc-globulin

  • ELISA Kit for Vitamin D Binding Protein (DBP) Packages (Simulation)
  • ELISA Kit for Vitamin D Binding Protein (DBP) Packages (Simulation)
  • ELISA Kit for Vitamin D Binding Protein (DBP) Results demonstration
  • SEB810Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Vitamin D Binding Protein (DBP)

This assay has high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP).
No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.

Recovery of the ELISA Kit for Vitamin D Binding Protein (DBP)

Matrices listed below were spiked with certain level of recombinant Vitamin D Binding Protein (DBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Vitamin D Binding Protein (DBP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-103 98
EDTA plasma(n=5) 80-101 89
heparin plasma(n=5) 78-90 86

Precision of the ELISA Kit for Vitamin D Binding Protein (DBP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Vitamin D Binding Protein (DBP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin D Binding Protein (DBP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-98% 79-101% 91-103% 84-98%
EDTA plasma(n=5) 83-92% 91-101% 92-101% 84-102%
heparin plasma(n=5) 93-104% 85-104% 91-99% 92-101%

Stability of the ELISA Kit for Vitamin D Binding Protein (DBP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Vitamin D Binding Protein (DBP)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Vitamin D Binding Protein (DBP)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB810Hu02 Recombinant Vitamin D Binding Protein (DBP) Positive Control; Immunogen; SDS-PAGE; WB.
APB810Hu61 Active Vitamin D Binding Protein (DBP) Cell culture; Activity Assays.
EPB810Hu61 Eukaryotic Vitamin D Binding Protein (DBP) Positive Control; Immunogen; SDS-PAGE; WB.
RPB810Hu03 Recombinant Vitamin D Binding Protein (DBP) Positive Control; Immunogen; SDS-PAGE; WB.
RPB810Hu01 Recombinant Vitamin D Binding Protein (DBP) Positive Control; Immunogen; SDS-PAGE; WB.
PAB810Hu03 Polyclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
PAB810Hu02 Polyclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
PAB810Hu01 Polyclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
LAB810Hu71 Biotin-Linked Polyclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC.
MAB810Hu22 Monoclonal Antibody to Vitamin D Binding Protein (DBP) WB,IHC
MAB810Hu24 Monoclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
MAB810Hu25 Monoclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
MAB810Hu27 Monoclonal Antibody to Vitamin D Binding Protein (DBP) WB.
MAB810Hu21 Monoclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
MAB810Hu23 Monoclonal Antibody to Vitamin D Binding Protein (DBP) WB; IHC; ICC; IP.
SEB810Hu ELISA Kit for Vitamin D Binding Protein (DBP) Enzyme-linked immunosorbent assay for Antigen Detection.
AEB810Hu ELISA Kit for Anti-Vitamin D Binding Protein Antibody (Anti-DBP) Enzyme-linked immunosorbent assay for Antibody Detection.
SCB810Hu CLIA Kit for Vitamin D Binding Protein (DBP) Chemiluminescent immunoassay for Antigen Detection.
LMB810Hu Multiplex Assay Kit for Vitamin D Binding Protein (DBP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB810Hu01 ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK