ELISA Kit for Complement Receptor 2 (CD21)

CR2; C3DR; Complement Component(3d/Epstein Barr Virus)Receptor 2; Complement C3d receptor; Epstein-Barr virus receptor; EBV receptor

  • ELISA Kit for Complement Receptor 2 (CD21) Packages (Simulation)
  • ELISA Kit for Complement Receptor 2 (CD21) Packages (Simulation)
  • ELISA Kit for Complement Receptor 2 (CD21) Results demonstration
  • SEA750Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Complement Receptor 2 (CD21)

This assay has high sensitivity and excellent specificity for detection of Complement Receptor 2 (CD21).
No significant cross-reactivity or interference between Complement Receptor 2 (CD21) and analogues was observed.

Recovery of the ELISA Kit for Complement Receptor 2 (CD21)

Matrices listed below were spiked with certain level of recombinant Complement Receptor 2 (CD21) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Receptor 2 (CD21) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 80-92 88
heparin plasma(n=5) 83-102 95

Precision of the ELISA Kit for Complement Receptor 2 (CD21)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Receptor 2 (CD21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Receptor 2 (CD21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Complement Receptor 2 (CD21)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Receptor 2 (CD21) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-98% 86-94% 87-104% 96-103%
EDTA plasma(n=5) 80-89% 92-104% 82-99% 83-91%
heparin plasma(n=5) 90-104% 82-95% 88-98% 87-94%

Stability of the ELISA Kit for Complement Receptor 2 (CD21)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Complement Receptor 2 (CD21)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Complement Receptor 2 (CD21)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 2 (CD21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 2 (CD21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 2 (CD21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Receptor 2 (CD21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA750Hu01 Recombinant Complement Receptor 2 (CD21) Positive Control; Immunogen; SDS-PAGE; WB.
PAA750Hu01 Polyclonal Antibody to Complement Receptor 2 (CD21) WB; IHC; ICC; IP.
FAA750Hu01 Anti-Complement Receptor 2 (CD21) Polyclonal Antibody Flow cytometry.
MAA750Hu23 Monoclonal Antibody to Complement Receptor 2 (CD21) WB; IHC; ICC; IP.
MAA750Hu25 Monoclonal Antibody to Complement Receptor 2 (CD21) WB; IHC; ICC; IP.
MAA750Hu21 Monoclonal Antibody to Complement Receptor 2 (CD21) WB; IHC; ICC; IP.
MAA750Hu24 Monoclonal Antibody to Complement Receptor 2 (CD21) WB; IHC; ICC; IP.
MAA750Hu22 Monoclonal Antibody to Complement Receptor 2 (CD21) WB; IHC; ICC; IP.
SEA750Hu ELISA Kit for Complement Receptor 2 (CD21) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA750Hu Multiplex Assay Kit for Complement Receptor 2 (CD21) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK