ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No.SEC419Hu
Sample typeSerum, plasma and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range125-8000pg/mL The standard curve concentrations used for the ELISA’s were 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 57pg/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Cathelicidin Antimicrobial Peptide (CAMP).
No significant cross-reactivity or interference between Cathelicidin Antimicrobial Peptide (CAMP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cathelicidin Antimicrobial Peptide (CAMP) and the recovery rates were calculated by comparing the measured value to the expected amount of Cathelicidin Antimicrobial Peptide (CAMP) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)82-10189
EDTA plasma(n=5)95-10399
heparin plasma(n=5)87-10197
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathelicidin Antimicrobial Peptide (CAMP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathelicidin Antimicrobial Peptide (CAMP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cathelicidin Antimicrobial Peptide (CAMP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)81-98%81-94%79-94%79-92%
EDTA plasma(n=5)89-102%85-94%85-101%85-99%
heparin plasma(n=5)92-101%78-98%97-105%88-101%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide (CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Package and Components
Reagent Preparation
Results demonstration
Typical Standard Curve
Certificate
Download
Free use and Guarantees
Free trial
In case it is difficult to pre-evaluate experiment result because of specific test samples or other reasons, free trial can be offered.
If the budget is really limited or other special situation, you can apply the free products.
Guarantees
Unconditionally return policy within 72 hours.
For any complaint, we promise to reply within one work day, resolve the problem within three work days. Implementing initial consultation system in order to provide satisfactory service for all the customers. Customers can unconditionally to request a charge back or refund.
Benchmark price
MagazineReference
Archives of Dermatological Research Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters    Read more(SpringerLink: t07u24t43r357l15)
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
CPC419Hu21OVA Conjugated Cathelicidin Antimicrobial Peptide (CAMP)SDS-PAGE; WB; ELISA; IP.
MAC419Hu22Monoclonal Antibody to Cathelicidin Antimicrobial Peptide (CAMP)WB, ICC, IHC-P, IHC-F, ELISA
PAC419Hu01Polyclonal Antibody to Cathelicidin Antimicrobial Peptide (CAMP)WB, ICC, IHC-P, IHC-F, ELISA
PAC419Hu02Polyclonal Antibody to Cathelicidin Antimicrobial Peptide (CAMP)WB, ICC, IHC-P, IHC-F, ELISA
PAC419Hu71Biotin-Linked Antibody to Cathelicidin Antimicrobial Peptide (CAMP)IHC;WB;ELISA
RPC419Hu01Recombinant Cathelicidin Antimicrobial Peptide (CAMP)SDS-PAGE; WB; ELISA; IP.
SEC419HuELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)Enzyme-linked immunosorbent assay