ELISA Kit for Endothelin 1 (EDN1)

ET1; PPET1; Preproendothelin-1; Big endothelin-1

  • ELISA Kit for Endothelin 1 (EDN1) Packages (Simulation)
  • ELISA Kit for Endothelin 1 (EDN1) Packages (Simulation)
  • ELISA Kit for Endothelin 1 (EDN1) Results demonstration
  • CEA482Po.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Endothelin 1 (EDN1)

This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1).
No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.

Recovery of the ELISA Kit for Endothelin 1 (EDN1)

Matrices listed below were spiked with certain level of recombinant Endothelin 1 (EDN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelin 1 (EDN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 91
EDTA plasma(n=5) 92-105 96
heparin plasma(n=5) 86-94 90

Precision of the ELISA Kit for Endothelin 1 (EDN1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Endothelin 1 (EDN1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelin 1 (EDN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 92-103% 95-103% 92-101%
EDTA plasma(n=5) 83-105% 78-93% 82-91% 84-95%
heparin plasma(n=5) 99-105% 95-105% 90-98% 85-94%

Stability of the ELISA Kit for Endothelin 1 (EDN1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Endothelin 1 (EDN1)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Endothelin 1 (EDN1)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 1 (EDN1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) and unlabeled Endothelin 1 (EDN1) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample.

Related products

Catalog No. Organism species: Sus scrofa; Porcine (Pig) Applications (RESEARCH USE ONLY!)
RPA482Po01 Recombinant Endothelin 1 (EDN1) Positive Control; Immunogen; SDS-PAGE; WB.
CPA482Po11 BSA Conjugated Endothelin 1 (EDN1) Immunogen; SDS-PAGE; WB.
PAA482Po08 Polyclonal Antibody to Endothelin 1 (EDN1) WB; IHC; ICC; IP.
PAA482Po01 Polyclonal Antibody to Endothelin 1 (EDN1) WB; IHC; ICC; IP.
LAA482Po81 FITC-Linked Polyclonal Antibody to Endothelin 1 (EDN1) WB; IHC; ICC; IF.
LAA482Po71 Biotin-Linked Polyclonal Antibody to Endothelin 1 (EDN1) WB; IHC; ICC.
MAA482Po21 Monoclonal Antibody to Endothelin 1 (EDN1) WB; IHC; ICC; IP.
CEA482Po ELISA Kit for Endothelin 1 (EDN1) Enzyme-linked immunosorbent assay for Antigen Detection.
GUESTBOOK