ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

CG-B; CGB3; hCGB; HCG; CGb5; CGb7; CGb8; Choriogonadotropin subunit beta

  • ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Packages (Simulation)
  • ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Packages (Simulation)
  • ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Results demonstration
  • CED215Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

This assay has high sensitivity and excellent specificity for detection of Chorionic Gonadotropin Beta Polypeptide (CGb).
No significant cross-reactivity or interference between Chorionic Gonadotropin Beta Polypeptide (CGb) and analogues was observed.

Recovery of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

Matrices listed below were spiked with certain level of recombinant Chorionic Gonadotropin Beta Polypeptide (CGb) and the recovery rates were calculated by comparing the measured value to the expected amount of Chorionic Gonadotropin Beta Polypeptide (CGb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-96 93
EDTA plasma(n=5) 89-99 93
heparin plasma(n=5) 99-105 102

Precision of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chorionic Gonadotropin Beta Polypeptide (CGb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chorionic Gonadotropin Beta Polypeptide (CGb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-98% 78-94% 85-104% 93-105%
EDTA plasma(n=5) 78-98% 78-99% 83-102% 90-99%
heparin plasma(n=5) 78-101% 81-94% 79-91% 91-105%

Stability of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Chorionic Gonadotropin Beta Polypeptide (CGb) and unlabeled Chorionic Gonadotropin Beta Polypeptide (CGb) (Standards or samples) with the pre-coated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the sample.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPD215Hu01 Recombinant Chorionic Gonadotropin Beta Polypeptide (CGb) Positive Control; Immunogen; SDS-PAGE; WB.
PAD215Hu01 Polyclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb) WB; IHC; ICC; IP.
MAD215Hu21 Monoclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb) WB; IHC; ICC; IP.
MAD215Hu22 Monoclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb) WB; IHC; ICC; IP.
MAD215Hu23 Monoclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb) WB; IHC; ICC; IP.
SED215Hu ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Enzyme-linked immunosorbent assay for Antigen Detection.
CED215Hu ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Enzyme-linked immunosorbent assay for Antigen Detection.
AED215Hu ELISA Kit for Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) Enzyme-linked immunosorbent assay for Antibody Detection.
KSD215Hu01 ELISA Kit DIY Materials for Chorionic Gonadotropin Beta Polypeptide (CGb) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK