ELISA Kit DIY Materials for Nitrotyrosine (NT)

3-Nitro-L-Tyrosine; 3-Nitrotyrosine

  • ELISA Kit DIY Materials for Nitrotyrosine (NT) Packages (Simulation)
  • ELISA Kit DIY Materials for Nitrotyrosine (NT) Packages (Simulation)
  • KSB863Ge11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the ELISA Kit DIY Materials for Nitrotyrosine (NT)

The Abs in the kit have high sensitivity and excellent specificity for detection of Nitrotyrosine (NT). No significant cross-reactivity or interference between Nitrotyrosine (NT) and analogues was observed.

USAGE of the ELISA Kit DIY Materials for Nitrotyrosine (NT)

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE of the ELISA Kit DIY Materials for Nitrotyrosine (NT)

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

SUPPORT PACK of the ELISA Kit DIY Materials for Nitrotyrosine (NT)

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