CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

  • CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1) Results demonstration
  • SCA124Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.

Recovery of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 1 (TGFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 1 (TGFb1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-94 82
EDTA plasma(n=5) 82-103 85
heparin plasma(n=5) 86-103 98

Precision of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 1 (TGFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 81-94% 79-103% 87-96%
EDTA plasma(n=5) 98-105% 83-99% 97-105% 95-103%
heparin plasma(n=5) 84-102% 78-95% 96-103% 90-104%

Stability of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)

The microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Beta 1 (TGFb1) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA124Mu02 Recombinant Transforming Growth Factor Beta 1 (TGFb1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA124Mu01 Recombinant Transforming Growth Factor Beta 1 (TGFb1) Positive Control; Immunogen; SDS-PAGE; WB.
EPA124Mu61 Eukaryotic Transforming Growth Factor Beta 1 (TGFb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA124Mu01 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1) WB; IHC; ICC; IP.
PAA124Mu02 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1) WB; IHC; ICC; IP.
LAA124Mu81 FITC-Linked Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1) WB; IHC; ICC; IF.
LAA124Mu71 Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1) WB; IHC; ICC.
SEA124Mu ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA124Mu Mini Samples ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA124Mu CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1) Chemiluminescent immunoassay for Antigen Detection.
KSA124Mu01 ELISA Kit DIY Materials for Transforming Growth Factor Beta 1 (TGFb1) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK