CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

MYBPCC; MYBPCS; C-protein, skeletal muscle slow isoform

  • CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Packages (Simulation)
  • CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Packages (Simulation)
  • CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Results demonstration
  • SCD413Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

This assay has high sensitivity and excellent specificity for detection of Myosin Binding Protein C, Slow Type (MYBPC1).
No significant cross-reactivity or interference between Myosin Binding Protein C, Slow Type (MYBPC1) and analogues was observed.

Precision of the CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Binding Protein C, Slow Type (MYBPC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Binding Protein C, Slow Type (MYBPC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1)

The microplate provided in this kit has been pre-coated with an antibody specific to Myosin Binding Protein C, Slow Type (MYBPC1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myosin Binding Protein C, Slow Type (MYBPC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myosin Binding Protein C, Slow Type (MYBPC1) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPD413Mu01 Recombinant Myosin Binding Protein C, Slow Type (MYBPC1) Positive Control; Immunogen; SDS-PAGE; WB.
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PAD413Mu01 Polyclonal Antibody to Myosin Binding Protein C, Slow Type (MYBPC1) WB; IHC; ICC; IP.
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SED413Mu ELISA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD413Mu CLIA Kit for Myosin Binding Protein C, Slow Type (MYBPC1) Chemiluminescent immunoassay for Antigen Detection.
LMD413Mu Multiplex Assay Kit for Myosin Binding Protein C, Slow Type (MYBPC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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