CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

CLG3; Collagenase 3

  • CLIA Kit for Matrix Metalloproteinase 13 (MMP13) Packages (Simulation)
  • CLIA Kit for Matrix Metalloproteinase 13 (MMP13) Packages (Simulation)
  • CLIA Kit for Matrix Metalloproteinase 13 (MMP13) Results demonstration
  • SCA099Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 13 (MMP13).
No significant cross-reactivity or interference between Matrix Metalloproteinase 13 (MMP13) and analogues was observed.

Recovery of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 13 (MMP13) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 13 (MMP13) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 101
heparin plasma(n=5) 85-101 92

Precision of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 13 (MMP13) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-98% 79-92% 83-94% 81-102%
heparin plasma(n=5) 91-98% 81-93% 82-92% 81-98%

Stability of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 13 (MMP13). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 13 (MMP13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 13 (MMP13) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA099Hu01 Recombinant Matrix Metalloproteinase 13 (MMP13) Positive Control; Immunogen; SDS-PAGE; WB.
RPA099Hu02 Recombinant Matrix Metalloproteinase 13 (MMP13) Positive Control; Immunogen; SDS-PAGE; WB.
APA099Hu02 Active Matrix Metalloproteinase 13 (MMP13) Cell culture; Activity Assays.
APA099Hu01 Active Matrix Metalloproteinase 13 (MMP13) Cell culture; Activity Assays.
PAA099Hu01 Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC; IP.
PAA099Hu02 Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC; IP.
LAA099Hu71 Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC.
MAA099Hu22 Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC; IP.
HEA099Hu High Sensitive ELISA Kit for Matrix Metalloproteinase 13 (MMP13) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA099Hu ELISA Kit for Matrix Metalloproteinase 13 (MMP13) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA099Hu CLIA Kit for Matrix Metalloproteinase 13 (MMP13) Chemiluminescent immunoassay for Antigen Detection.
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