CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

STMY2; SL2; Stromelysin 2; Transin-2

  • CLIA Kit for Matrix Metalloproteinase 10 (MMP10) Packages (Simulation)
  • CLIA Kit for Matrix Metalloproteinase 10 (MMP10) Packages (Simulation)
  • CLIA Kit for Matrix Metalloproteinase 10 (MMP10) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10).
No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.

Recovery of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 10 (MMP10) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 10 (MMP10) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-102 91
EDTA plasma(n=5) 84-102 99
heparin plasma(n=5) 98-105 102

Precision of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 10 (MMP10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 81-99% 83-99% 96-104%
EDTA plasma(n=5) 79-98% 87-101% 80-95% 95-103%
heparin plasma(n=5) 91-98% 94-104% 92-101% 79-101%

Stability of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Matrix Metalloproteinase 10 (MMP10)

The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 10 (MMP10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 10 (MMP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 10 (MMP10) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA098Mu01 Recombinant Matrix Metalloproteinase 10 (MMP10) Positive Control; Immunogen; SDS-PAGE; WB.
RPA098Mu02 Recombinant Matrix Metalloproteinase 10 (MMP10) Positive Control; Immunogen; SDS-PAGE; WB.
PAA098Mu01 Polyclonal Antibody to Matrix Metalloproteinase 10 (MMP10) WB; IHC; ICC; IP.
PAA098Mu02 Polyclonal Antibody to Matrix Metalloproteinase 10 (MMP10) WB; IHC; ICC; IP.
SEA098Mu ELISA Kit for Matrix Metalloproteinase 10 (MMP10) Enzyme-linked immunosorbent assay for Antigen Detection.
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LMA098Mu Multiplex Assay Kit for Matrix Metalloproteinase 10 (MMP10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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