CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine

  • CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Packages (Simulation)
  • CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Packages (Simulation)
  • CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Results demonstration
  • SCA095Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a).
No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) and analogues was observed.

Recovery of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

Matrices listed below were spiked with certain level of recombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a) and the recovery rates were calculated by comparing the measured value to the expected amount of Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-99 89
EDTA plasma(n=5) 80-90 85
heparin plasma(n=5) 92-99 96

Precision of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-97% 93-105% 83-90% 98-105%
EDTA plasma(n=5) 85-104% 80-94% 99-105% 80-102%
heparin plasma(n=5) 83-101% 96-104% 78-104% 96-105%

Stability of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Inflammatory Protein 3 Alpha (MIP3a) level in the sample or standard.;

Related products

Catalog No. Organism species: Rattus norvegicus (Rat) Applications (RESEARCH USE ONLY!)
RPA095Ra01 Recombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a) Positive Control; Immunogen; SDS-PAGE; WB.
PAA095Ra01 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a) WB; IHC; ICC; IP.
MAA095Ra21 Monoclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a) WB; IHC; ICC; IP.
SEA095Ra ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA095Ra CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Chemiluminescent immunoassay for Antigen Detection.
LMA095Ra Multiplex Assay Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA095Ra01 ELISA Kit DIY Materials for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK