CLIA Kit for Kidney Injury Molecule 1 (Kim1)

HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

  • CLIA Kit for Kidney Injury Molecule 1 (Kim1) Packages (Simulation)
  • CLIA Kit for Kidney Injury Molecule 1 (Kim1) Packages (Simulation)
  • CLIA Kit for Kidney Injury Molecule 1 (Kim1) Results demonstration
  • SCA785Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Kidney Injury Molecule 1 (Kim1)

This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1).
No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Precision of the CLIA Kit for Kidney Injury Molecule 1 (Kim1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the CLIA Kit for Kidney Injury Molecule 1 (Kim1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Kidney Injury Molecule 1 (Kim1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Kidney Injury Molecule 1 (Kim1)

The microplate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Kidney Injury Molecule 1 (Kim1) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA785Hu01 Recombinant Kidney Injury Molecule 1 (Kim1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA785Hu02 Recombinant Kidney Injury Molecule 1 (Kim1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA785Hu03 Recombinant Kidney Injury Molecule 1 (Kim1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA785Hu01 Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1) WB; IHC; ICC; IP.
PAA785Hu02 Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1) WB; IHC; ICC; IP.
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LAA785Hu81 FITC-Linked Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1) WB; IHC; ICC; IF.
LAA785Hu71 Biotin-Linked Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1) WB; IHC; ICC.
MAA785Hu22 Monoclonal Antibody to Kidney Injury Molecule 1 (Kim1) WB; IHC; ICC; IP.
SEA785Hu ELISA Kit for Kidney Injury Molecule 1 (Kim1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA785Hu CLIA Kit for Kidney Injury Molecule 1 (Kim1) Chemiluminescent immunoassay for Antigen Detection.
KSA785Hu01 ELISA Kit DIY Materials for Kidney Injury Molecule 1 (Kim1) Main materials for "Do It (ELISA Kit) Yourself".
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