CLIA Kit for Enolase 1 (ENO1)

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

  • CLIA Kit for Enolase 1 (ENO1) Packages (Simulation)
  • CLIA Kit for Enolase 1 (ENO1) Packages (Simulation)
  • CLIA Kit for Enolase 1 (ENO1) Results demonstration
  • SCB449Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Enolase 1 (ENO1)

This assay has high sensitivity and excellent specificity for detection of Enolase 1 (ENO1).
No significant cross-reactivity or interference between Enolase 1 (ENO1) and analogues was observed.

Recovery of the CLIA Kit for Enolase 1 (ENO1)

Matrices listed below were spiked with certain level of recombinant Enolase 1 (ENO1) and the recovery rates were calculated by comparing the measured value to the expected amount of Enolase 1 (ENO1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 78-102 90
heparin plasma(n=5) 86-101 94

Precision of the CLIA Kit for Enolase 1 (ENO1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase 1 (ENO1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase 1 (ENO1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Enolase 1 (ENO1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Enolase 1 (ENO1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-92% 91-99% 97-105% 79-102%
EDTA plasma(n=5) 83-103% 87-94% 81-103% 93-101%
heparin plasma(n=5) 94-102% 93-103% 78-103% 80-104%

Stability of the CLIA Kit for Enolase 1 (ENO1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Enolase 1 (ENO1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Enolase 1 (ENO1)

The microplate provided in this kit has been pre-coated with an antibody specific to Enolase 1 (ENO1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Enolase 1 (ENO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Enolase 1 (ENO1) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB449Hu03 Recombinant Enolase 1 (ENO1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB449Hu02 Recombinant Enolase 1 (ENO1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB449Hu01 Recombinant Enolase 1 (ENO1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB449Hu02 Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
PAB449Hu03 Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
PAB449Hu01 Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
LAB449Hu71 Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC.
MAB449Hu21 Monoclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
MAB449Hu22 Monoclonal Antibody to Enolase 1 (ENO1) WB; IHC.
SEB449Hu ELISA Kit for Enolase 1 (ENO1) Enzyme-linked immunosorbent assay for Antigen Detection.
AEB449Hu ELISA Kit for Anti-Enolase 1 Antibody (Anti-ENO1) Enzyme-linked immunosorbent assay for Antibody Detection.
SCB449Hu CLIA Kit for Enolase 1 (ENO1) Chemiluminescent immunoassay for Antigen Detection.
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