CLIA Kit for Cathepsin D (CTSD)

CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis,Neuronal 10

  • CLIA Kit for Cathepsin D (CTSD) Packages (Simulation)
  • CLIA Kit for Cathepsin D (CTSD) Packages (Simulation)
  • CLIA Kit for Cathepsin D (CTSD) Results demonstration
  • SCB280Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Cathepsin D (CTSD)

This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD).
No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.

Recovery of the CLIA Kit for Cathepsin D (CTSD)

Matrices listed below were spiked with certain level of recombinant Cathepsin D (CTSD) and the recovery rates were calculated by comparing the measured value to the expected amount of Cathepsin D (CTSD) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-90 87
EDTA plasma(n=5) 79-92 82
heparin plasma(n=5) 90-104 96

Precision of the CLIA Kit for Cathepsin D (CTSD)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Cathepsin D (CTSD)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cathepsin D (CTSD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-101% 80-97% 86-101% 79-99%
EDTA plasma(n=5) 78-94% 92-99% 94-101% 79-88%
heparin plasma(n=5) 78-97% 83-97% 80-96% 89-101%

Stability of the CLIA Kit for Cathepsin D (CTSD)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Cathepsin D (CTSD)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Cathepsin D (CTSD)

The microplate provided in this kit has been pre-coated with an antibody specific to Cathepsin D (CTSD). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cathepsin D (CTSD) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPB280Mu01 Recombinant Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB280Mu02 Recombinant Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
PAB280Mu02 Polyclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
PAB280Mu01 Polyclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
SEB280Mu ELISA Kit for Cathepsin D (CTSD) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB280Mu CLIA Kit for Cathepsin D (CTSD) Chemiluminescent immunoassay for Antigen Detection.
LMB280Mu Multiplex Assay Kit for Cathepsin D (CTSD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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