CLIA Kit for Apolipoprotein B (APOB)

Apo-B; FLDB; Apo B-100; Apo B-48

  • CLIA Kit for Apolipoprotein B (APOB) Packages (Simulation)
  • CLIA Kit for Apolipoprotein B (APOB) Packages (Simulation)
  • CLIA Kit for Apolipoprotein B (APOB) Results demonstration
  • SCC003Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Apolipoprotein B (APOB)

This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB).
No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.

Recovery of the CLIA Kit for Apolipoprotein B (APOB)

Matrices listed below were spiked with certain level of recombinant Apolipoprotein B (APOB) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein B (APOB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-99 96
EDTA plasma(n=5) 83-101 92
heparin plasma(n=5) 93-104 98

Precision of the CLIA Kit for Apolipoprotein B (APOB)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Apolipoprotein B (APOB)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein B (APOB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 97-104% 79-96% 99-105%
EDTA plasma(n=5) 79-102% 79-101% 80-99% 98-105%
heparin plasma(n=5) 79-101% 95-103% 87-97% 85-97%

Stability of the CLIA Kit for Apolipoprotein B (APOB)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Apolipoprotein B (APOB)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Apolipoprotein B (APOB)

The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein B (APOB) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
NPC003Hu01 Native Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu02 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu01 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
PAC003Hu02 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
PAC003Hu01 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Hu81 FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IF.
LAC003Hu71 Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
MAC003Hu22 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
MAC003Hu21 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
SEC003Hu ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
HEC003Hu High Sensitive ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC003Hu CLIA Kit for Apolipoprotein B (APOB) Chemiluminescent immunoassay for Antigen Detection.
GUESTBOOK