Biotin-Linked Polyclonal Antibody to Immunoglobulin G (IgG)
- Product No.LAA544Ov71
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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SPECIFITY of the Biotin-Linked Polyclonal Antibody to Immunoglobulin G (IgG)
The antibody is a rabbit polyclonal antibody raised against IgG. It has been selected for its ability to recognize IgG in immunohistochemical staining and western blotting.
USAGE of the Biotin-Linked Polyclonal Antibody to Immunoglobulin G (IgG)
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE of the of the Biotin-Linked Polyclonal Antibody to Immunoglobulin G (IgG)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Biotin-Linked Polyclonal Antibody to Immunoglobulin G (IgG)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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