Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3)
Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
- Product No.LAA920Ge71
- Organism SpeciesPan-species (General) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download n/a
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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SPECIFITY of the Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3)
The antibody is a rabbit polyclonal antibody raised against VD3. It has been selected for its ability to recognize VD3 in immunohistochemical staining and western blotting.
USAGE of the Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE of the of the Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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