Polyclonal Antibody to Osteonectin (ON)

SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine

  • Polyclonal Antibody to Osteonectin (ON) Packages (Simulation)
  • Polyclonal Antibody to Osteonectin (ON) Packages (Simulation)
  • Polyclonal Antibody to Osteonectin (ON) DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat ON Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Osteonectin (ON) DAB staining on IHC-P;
    Samples: Rat Testis Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Rat ON Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Osteonectin (ON) DAB staining on IHC-P;
    Sample: Rat Testis Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat ON Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Osteonectin (ON) DAB staining on IHC-P;
    Sample: Rat Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat ON Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA791Ra01.jpg Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Lung lysate; Lane3: Rat Placenta lysate Primary Ab: 0.4µg/ml Rabbit Anti-Rat ON Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Osteonectin (ON)

The antibody is a rabbit polyclonal antibody raised against ON. It has been selected for its ability to recognize ON in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Osteonectin (ON)

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Osteonectin (ON)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Osteonectin (ON)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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