Polyclonal Antibody to Nucleophosmin 1 (NPM1)
B23; NPM; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38
- Product No.PAC664Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC664Hu02-Recombinant Nucleophosmin 1 (NPM1)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Vector Red staining on IHC-P;
Samples: Human Thyroid Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
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Figure. Western Blot; Sample: Recombinant NPM1, Human.
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Testis lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human U2OS cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human K562 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human NPM1 Polyclonal Antibody (Catalog PAC664Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
The antibody is a rabbit polyclonal antibody raised against NPM1. It has been selected for its ability to recognize NPM1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPC664Hu01 | Recombinant Nucleophosmin 1 (NPM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC664Hu02 | Recombinant Nucleophosmin 1 (NPM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC664Hu02 | Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC; IP. |
| PAC664Hu01 | Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC; IP. |
| LAC664Hu71 | Biotin-Linked Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC. |
| MAC664Hu22 | Monoclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC; IP. |
| SEC664Hu | ELISA Kit for Nucleophosmin 1 (NPM1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC664Hu | Multiplex Assay Kit for Nucleophosmin 1 (NPM1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |