Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
HGPRT; HGPRTase; HPRT; Lesch-Nyhan Syndrome; Hypoxanthine-guanine phosphoribosyltransferase
- Product No.PAA717Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA717Hu01-Recombinant Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreRattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 95
For more details, please contact local distributors!US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Colon Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human HPRT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant HPRT1, Human.
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Figure. Western Blot; Sample: Lane1: Rat Liver Tissue; Lane2: Rat Brain Tissue; Lane3: Human Hela Cells; Lane4: Human HepG2 Cells; Lane5: Human A549 Cells.
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HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HPRT1 Polyclonal Antibody (Catalog # PAA717Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
The antibody is a rabbit polyclonal antibody raised against HPRT1. It has been selected for its ability to recognize HPRT1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
Western blotting: 0.5-5µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA717Hu01 | Recombinant Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA717Hu01 | Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) | WB; IHC; ICC; IP. |
| FAA717Hu01 | Anti-Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Polyclonal Antibody | Flow cytometry. |
| MAA717Hu22 | Monoclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) | WB; IHC; ICC; IP. |
| SEA717Hu | ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA717Hu | Multiplex Assay Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |