Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- Product No.PAB081Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB081Hu01-Recombinant Heat Shock 70kDa Protein 1A (HSPA1A)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 100
For more details, please contact local distributors!US$ 232
For more details, please contact local distributors! US$ 332
For more details, please contact local distributors! US$ 830
For more details, please contact local distributors! US$ 3320
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human HSPA1A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure. DAB staining on IHC-P;
Samples: Human Esophagus Cancer Tissue. -
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure: FITC staining on IHC-P;
Sample: A549 cells.
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Figure. Western Blot; Sample: Recombinant HSPA1A, Human.
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Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Heart lysate; Lane4: Mouse Liver lysate; Lane5: Mouse Cerebrum lysate
Primary Ab: 1µg/mL Rabbit Anti-Human HSPA1A Ab
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human HSPA1A Polyclonal Antibody (Catalog PAB081Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
The antibody is a rabbit polyclonal antibody raised against HSPA1A. It has been selected for its ability to recognize HSPA1A in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service