Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
- Product No.MAB932Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)3-2#
- Ig Isotype N/A
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB932Hu01-Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsLoading Control of WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 125
For more details, please contact local distributors!US$ 293
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For more details, please contact local distributors! US$ 4180
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Liver Tissue Primary Ab: 30µg/ml Mouse Anti-Human GAPDH Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 30µg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Thyroid cancer Tissue
Primary Ab: 30µg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Figure. Western Blot; Sample: Recombinant GAPDH, Human.
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Western Blot; Samples: Lane1: Human Liver lysate; Lane2: Rat Liver lysate; Lane3: Mouse Liver lysate;
Primary Ab: 1µg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
The antibody is a mouse monoclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service