Monoclonal Antibody to Cluster Of Differentiation 147 (CD147)
EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
- Product No.MAB540Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C20
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB540Hu01-Recombinant Cluster Of Differentiation 147 (CD147)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreRattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Adrenal gland Tissue; Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Adrenal gland Tissue
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human CD147 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: Lane1: Rat Kidney lysate; Lane2: Hela cell lysate Primary Ab: 1µg/ml Mouse Anti-Human CD147 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Cluster Of Differentiation 147 (CD147)
The antibody is a mouse monoclonal antibody raised against CD147. It has been selected for its ability to recognize CD147 in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Cluster Of Differentiation 147 (CD147)
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Cluster Of Differentiation 147 (CD147)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Cluster Of Differentiation 147 (CD147)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service