Active Vascular Endothelial Growth Factor 165 (VEGF165)

  • Active Vascular Endothelial Growth Factor 165 (VEGF165) Packages (Simulation)
  • Active Vascular Endothelial Growth Factor 165 (VEGF165) Packages (Simulation)
  • Active Vascular Endothelial Growth Factor 165 (VEGF165) Figure. Gene Sequencing (Extract)
  • APB696Hu01.png Figure. SDS-PAGE
  • Active Vascular Endothelial Growth Factor 165 (VEGF165) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Vascular Endothelial Growth Factor 165 (VEGF165)

Figure. The binding activity of VEGF165 with VEGFR1.
Vascular endothelial growth factor 165 is one kind of isoforms of Vascular endothelial growth factor A (VEGFA). This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Besides, Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) has been identified as an interactor of VEGF165, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VEGF165 and recombinant human VEGFR1. Briefly, VEGF165 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VEGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VEGF165 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of VEGF165 and VEGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Vascular Endothelial Growth Factor 165 (VEGF165)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Vascular Endothelial Growth Factor 165 (VEGF165)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Vascular Endothelial Growth Factor 165 (VEGF165)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APB696Hu61 Active Vascular Endothelial Growth Factor 165 (VEGF165) Cell culture; Activity Assays.
APB696Hu01 Active Vascular Endothelial Growth Factor 165 (VEGF165) Cell culture; Activity Assays.
RPB696Hu01 Recombinant Vascular Endothelial Growth Factor 165 (VEGF165) Positive Control; Immunogen; SDS-PAGE; WB.
EPB696Hu61 Eukaryotic Vascular Endothelial Growth Factor 165 (VEGF165) Positive Control; Immunogen; SDS-PAGE; WB.
PAB696Hu01 Polyclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu21 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC
MAB696Hu22 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu25 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu24 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu23 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu26 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu27 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
MAB696Hu29 Monoclonal Antibody to Vascular Endothelial Growth Factor 165 (VEGF165) WB; IHC; ICC; IP.
FAB696Hu08 Anti-Vascular Endothelial Growth Factor 165 (VEGF165) Monoclonal Antibody Flow cytometry.
FAB696Hu06 Anti-Vascular Endothelial Growth Factor 165 (VEGF165) Monoclonal Antibody Flow cytometry.
FAB696Hu02 Anti-Vascular Endothelial Growth Factor 165 (VEGF165) Monoclonal Antibody Flow cytometry.
FAB696Hu04 Anti-Vascular Endothelial Growth Factor 165 (VEGF165) Monoclonal Antibody Flow cytometry.
SEB696Hu ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB696Hu Multiplex Assay Kit for Vascular Endothelial Growth Factor 165 (VEGF165) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK