Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

CD27; S152; T14; Tp55; CD27L receptor;

  • Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7) Packages (Simulation)
  • Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7) Packages (Simulation)
  • APB043Hu01.jpg SDS-PAGE
  • Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7) Figure. Western Blot; Sample: Recombinant TNFRSF7, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

TNFRSF7 (Tumor necrosis factor receptor superfamily member 7), also known as CD27 antigen, is a member of the TNF-receptor superfamily. This receptor is thought to be involved in tumor necrosis factor-activated receptor activity by binding with TNFs. Thus, a binding ELISA assay was conducted to detect the association of TNFRSF7 with TNFa. Briefly, recombinant human TNFRSF7 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF7 with TNFa was shown in Figure 1 and this effect was in a dose dependent manner.

USAGE of the Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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