Active Tubulin Beta (TUBb)
TUB-B; TUBB5; TUBB1; M40
- Product No.APB870Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.6
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant TUBb, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Tubulin Beta (TUBb)
Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MX1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Tubulin Beta (TUBb)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Tubulin Beta (TUBb)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Tubulin Beta (TUBb)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APB870Hu01||Active Tubulin Beta (TUBb)||Cell culture; Activity Assays.|
|RPB870Hu01||Recombinant Tubulin Beta (TUBb)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CAB870Hu01||Anti-Tubulin Beta (TUBb) Polyclonal Antibody||Loading Control of WB.|
|PAB870Hu01||Polyclonal Antibody to Tubulin Beta (TUBb)||WB; IHC; ICC; IP.|
|MAB870Hu21||Monoclonal Antibody to Tubulin Beta (TUBb)||WB; IHC; ICC; IP.|
|CAB870Hu22||Anti-Tubulin Beta (TUBb) Monoclonal Antibody||Loading Control of WB.|