Active S100 Calcium Binding Protein B (S100B)

S100-B; NEF; S100Beta; S-100 protein subunit beta

  • Active S100 Calcium Binding Protein B (S100B) Packages (Simulation)
  • Active S100 Calcium Binding Protein B (S100B) Packages (Simulation)
  • Active S100 Calcium Binding Protein B (S100B) Figure. Gene Sequencing (Extract)
  • APA567Hu01.png Figure. SDS-PAGE
  • Active S100 Calcium Binding Protein B (S100B) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active S100 Calcium Binding Protein B (S100B)

Figure. The binding activity of S100B with RAGE.
Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active S100 Calcium Binding Protein B (S100B)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active S100 Calcium Binding Protein B (S100B)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active S100 Calcium Binding Protein B (S100B)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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