Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine

  • Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Packages (Simulation)
  • Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Packages (Simulation)
  • APA855Mu01.png Figure. SDS-PAGE
  • Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

Figure. The binding activity of RANKL with PPARg.
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene. RANKL is a member of the tumor necrosis factor (TNF) cytokine family, it binds to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. It may also bind to osteoprotegerin, a protein secreted mainly by cells of the osteoblast lineage which is a potent inhibitor of osteoclast formation by preventing binding of RANKL to RANK. RANKL also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. Besides, Peroxisome Proliferator Activated Receptor Gamma (PPARg) has been identified as an interactor of RANKL, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse RANKL and recombinant mouse PPARg. Briefly, RANKL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then
transferred to PPARg-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RANKL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of RANKLand PPARg was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
EPA855Mu61 Eukaryotic Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Positive Control; Immunogen; SDS-PAGE; WB.
APA855Mu01 Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Cell culture; Activity Assays.
RPA855Mu01 Recombinant Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Positive Control; Immunogen; SDS-PAGE; WB.
APA855Mu61 Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Cell culture; Activity Assays.
RPA855Mu02 Recombinant Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Positive Control; Immunogen; SDS-PAGE; WB.
PAA855Mu01 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) WB; IHC; ICC; IP.
PAA855Mu02 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) WB; IHC; ICC; IP.
SEA855Mu ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Enzyme-linked immunosorbent assay for Antigen Detection.
GUESTBOOK