Active Parathyroid Hormone Related Protein (PTHrP)
PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone
- Product No.APA819Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point10.1
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 300
For more details, please contact local distributors!US$ 750
For more details, please contact local distributors! US$ 1500
For more details, please contact local distributors! US$ 4500
For more details, please contact local distributors! US$ 11250
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Gene sequencing
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant PTHrP, Mouse.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Parathyroid Hormone Related Protein (PTHrP)
PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Parathyroid Hormone Related Protein (PTHrP)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Parathyroid Hormone Related Protein (PTHrP)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Parathyroid Hormone Related Protein (PTHrP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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