Active Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • Active Nerve Growth Factor (NGF) Packages (Simulation)
  • Active Nerve Growth Factor (NGF) Packages (Simulation)
  • Active Nerve Growth Factor (NGF) Figure . Gene Sequencing (extract)
  • APA105Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Nerve Growth Factor (NGF)

Nerve Growth Factor (NGF) is the archetypal neurotrophin of a family of polypeptides .It has long occupied a critical role in developmental and adult neurobiology for its many important regulatory functions on the survival, growth and differentiation of nerve cells in the peripheral and central nervous system. The Caspase 3 (CASP3) is high affinity receptor for Nerve Growth Factor,thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse NGF and recombinant human CASP3. Briefly, NGF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CASP3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse NGF and recombinant human CASP3 was shown in Figure 1, the EC50 for this effect is 0.86 ug/mL.

USAGE of the Active Nerve Growth Factor (NGF)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Nerve Growth Factor (NGF)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Nerve Growth Factor (NGF)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
APA105Mu01 Active Nerve Growth Factor (NGF) Cell culture; Activity Assays.
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