Active Matrix Metalloproteinase 2 (MMP2)

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

  • Active Matrix Metalloproteinase 2 (MMP2) Packages (Simulation)
  • Active Matrix Metalloproteinase 2 (MMP2) Packages (Simulation)
  • APA100Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Matrix Metalloproteinase 2 (MMP2)

Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family .It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control .
Result: Gelatin hydrolysis by recombinant mouse MMP2 was shown in figure 1.
Figure 1. Hydrolysis of gelatin by recombinant mouse MMP2.

USAGE of the Active Matrix Metalloproteinase 2 (MMP2)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Matrix Metalloproteinase 2 (MMP2)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Matrix Metalloproteinase 2 (MMP2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA100Mu01 Recombinant Matrix Metalloproteinase 2 (MMP2) Positive Control; Immunogen; SDS-PAGE; WB.
APA100Mu01 Active Matrix Metalloproteinase 2 (MMP2) Cell culture; Activity Assays.
RPA100Mu02 Recombinant Matrix Metalloproteinase 2 (MMP2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA100Mu01 Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IP.
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