Active Interleukin 6 Receptor (IL6R)
CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80
- Product No.APB815Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point6.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 200
For more details, please contact local distributors!US$ 500
For more details, please contact local distributors! US$ 1000
For more details, please contact local distributors! US$ 3000
For more details, please contact local distributors! US$ 7500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE; Sample: Active recombinant IL6R, Mouse.
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Figure. Western Blot; Sample: Recombinant IL6R, Mouse.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 6 Receptor (IL6R)
IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.
USAGE of the Active Interleukin 6 Receptor (IL6R)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 6 Receptor (IL6R)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 6 Receptor (IL6R)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
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Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
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Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
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| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPB815Mu02 | Recombinant Interleukin 6 Receptor (IL6R) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB815Mu02 | Active Interleukin 6 Receptor (IL6R) | Cell culture; Activity Assays. |
| RPB815Mu01 | Recombinant Interleukin 6 Receptor (IL6R) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB815Mu02 | Polyclonal Antibody to Interleukin 6 Receptor (IL6R) | WB; IHC; ICC; IP. |
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| LAB815Mu71 | Biotin-Linked Polyclonal Antibody to Interleukin 6 Receptor (IL6R) | WB; IHC; ICC. |
| SEB815Mu | ELISA Kit for Interleukin 6 Receptor (IL6R) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB815Mu | Multiplex Assay Kit for Interleukin 6 Receptor (IL6R) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |