Active Interleukin 28B (IL28B)
IFNL3; Interferon,Lambda 3; Cytokine Zcyto22
- Product No.APC028Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point9.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. SDS-PAGE
- Figure. Western Blot
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 28B (IL28B)
Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Interleukin 28B (IL28B)
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 28B (IL28B)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 28B (IL28B)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APC028Hu01||Active Interleukin 28B (IL28B)||Cell culture; Activity Assays.|
|LAC028Hu81||FITC-Linked Polyclonal Antibody to Interleukin 28B (IL28B)||WB; IHC; ICC; IF.|
|LAC028Hu71||Biotin-Linked Polyclonal Antibody to Interleukin 28B (IL28B)||WB; IHC; ICC.|
|MAC028Hu22||Monoclonal Antibody to Interleukin 28B (IL28B)||WB; IHC; ICC; IP.|
|HEC028Hu||High Sensitive ELISA Kit for Interleukin 28B (IL28B)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEC028Hu||ELISA Kit for Interleukin 28B (IL28B)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC028Hu||CLIA Kit for Interleukin 28B (IL28B)||Chemiluminescent immunoassay for Antigen Detection.|