Active Interleukin 1 Receptor Antagonist (IL1RA)

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

  • Active Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Figure. Gene Sequencing (extract)
  • APA223Hu61.png Figure. SDS-PAGE
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Sample: Recombinant IL1RA, Human;
    Antibody: Rabbit Anti-Human IL1RA Ab (PAA223Hu06)
    Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Interleukin 1 Receptor Antagonist (IL1RA)

IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 (IL-1), preventing IL-1 from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has been identified as an interactor of IL-1RA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to IL-1a-coated microtiter wells and incubated for 2 h at 37℃. Wells were washed with PBST and incubated for 1 h with anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1a was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of IL-1RA with IL-1a.

USAGE of the Active Interleukin 1 Receptor Antagonist (IL1RA)

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Interleukin 1 Receptor Antagonist (IL1RA)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Interleukin 1 Receptor Antagonist (IL1RA)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APA223Hu01 Active Interleukin 1 Receptor Antagonist (IL1RA) Cell culture; Activity Assays.
RPA223Hu01 Recombinant Interleukin 1 Receptor Antagonist (IL1RA) Positive Control; Immunogen; SDS-PAGE; WB.
APA223Hu61 Active Interleukin 1 Receptor Antagonist (IL1RA) Cell culture; Activity Assays.
EPA223Hu61 Eukaryotic Interleukin 1 Receptor Antagonist (IL1RA) Positive Control; Immunogen; SDS-PAGE; WB.
PAA223Hu01 Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
PAA223Hu06 Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
LAA223Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC.
LAA223Hu81 FITC-Linked Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IF.
MAA223Hu26 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
MAA223Hu22 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
MAA223Hu24 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
MAA223Hu25 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
MAA223Hu21 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
MAA223Hu23 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
LAA223Hu72 Biotin-Linked Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC.
HEA223Hu High Sensitive ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA223Hu ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA223Hu Multiplex Assay Kit for Interleukin 1 Receptor Antagonist (IL1RA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA223Hu01 Antibody Pair for Interleukin 1 Receptor Antagonist (IL1RA) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA223Hu01 ELISA Kit DIY Materials for Interleukin 1 Receptor Antagonist (IL1RA) Main materials for "Do It(ELISA Kit) Yourself"
GUESTBOOK