Active Interleukin 1 Beta (IL1b)

IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin

  • Active Interleukin 1 Beta (IL1b) Packages (Simulation)
  • Active Interleukin 1 Beta (IL1b) Packages (Simulation)
  • Active Interleukin 1 Beta (IL1b) Gene sequencing
  • APA563Hu01.jpg SDS-PAGE
  • Active Interleukin 1 Beta (IL1b) Figure. Western Blot;
    Sample: Recombinant IL1b, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Interleukin 1 Beta (IL1b)

IL-1β (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It is produced by activated macrophages as a proprotein, and then proteolytically processed to an active form. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To detect the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA.
IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1.

USAGE of the Active Interleukin 1 Beta (IL1b)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Interleukin 1 Beta (IL1b)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Interleukin 1 Beta (IL1b)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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