Active Hexosaminidase A Alpha (HEXa)
TSD; Hex-A; Tay Sachs Disease; GM2 Gangliosidosis; Beta-Hexosaminidase Subunit Alpha; Beta-N-Acetylhexosaminidase Subunit Alpha; N-Acetyl-Beta-Glucosaminidase Alpha
- Product No.APA195Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point6.3
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant HEXa, Mouse.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Hexosaminidase A Alpha (HEXa)
Hexosaminidase A Alpha (HEXa) is a lysosomal enzyme. There are three predominant isoenzymes: hexosaminidase A, B and S. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines. The enzymes are composed of two alpha and/or beta subunits, which are coded by HEXA and HEXB genes, respectively. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit which contains a key residue, Arg-424 is able to hydrolyze GM2 gangliosides. Hexosaminidase A (alpha polypeptide) plays a critical role in the brain and spinal cord (central nervous system). Besides, Hexosaminidase B Beta (HEXB) has been identified as an interactor of HEXA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse HEXA and recombinant mouse HEXB. Briefly, HEXA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HEXB-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HEXA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of HEXA and HEXB was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Hexosaminidase A Alpha (HEXa)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Hexosaminidase A Alpha (HEXa)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Hexosaminidase A Alpha (HEXa)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Mus musculus (Mouse)||Applications (RESEARCH USE ONLY!)|
|APA195Mu01||Active Hexosaminidase A Alpha (HEXa)||Cell culture; Activity Assays.|
|RPA195Mu01||Recombinant Hexosaminidase A Alpha (HEXa)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA195Mu02||Recombinant Hexosaminidase A Alpha (HEXa)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA195Mu01||Polyclonal Antibody to Hexosaminidase A Alpha (HEXa)||WB; IHC; ICC; IP.|
|PAA195Mu02||Polyclonal Antibody to Hexosaminidase A Alpha (HEXa)||WB; IHC; ICC; IP.|
|SEA195Mu||ELISA Kit for Hexosaminidase A Alpha (HEXa)||Enzyme-linked immunosorbent assay for Antigen Detection.|