Active Growth Regulated Oncogene Beta (GROb)
CXCL2; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Chemokine(C-X-C-Motif)ligand 2
- Product No.APB603Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point6.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant GROb, Rat.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Growth Regulated Oncogene Beta (GROb)
Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Growth Regulated Oncogene Beta (GROb)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Growth Regulated Oncogene Beta (GROb)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Growth Regulated Oncogene Beta (GROb)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Rattus norvegicus (Rat)||Applications (RESEARCH USE ONLY!)|
|APB603Ra01||Active Growth Regulated Oncogene Beta (GROb)||Cell culture; Activity Assays.|
|RPB603Ra01||Recombinant Growth Regulated Oncogene Beta (GROb)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB603Ra01||Polyclonal Antibody to Growth Regulated Oncogene Beta (GROb)||WB; IHC; ICC; IP.|
|MAB603Ra21||Monoclonal Antibody to Growth Regulated Oncogene Beta (GROb)||WB; IHC; ICC; IP.|
|SEB603Ra||ELISA Kit for Growth Regulated Oncogene Beta (GROb)||Enzyme-linked immunosorbent assay for Antigen Detection.|